SOPs

SOP for measurement of ALD activity in extracts.

Cloning and heterologous expression of gluconeogenic enzymes from S. solfataricus in E. coli

SOP for the cultivation conditions of Plasmodium falciparum, including the protocol for synchronisation.

SOP for measurement of ENO activity in extracts.

SOP for measurement of G3PDH activity in extracts.

SOP for measurement of GAPDH activity in extracts.

SOP for measurement of glucose transport activity in intact trophozoites.

SOP for measurement of HK activity in extracts.

SOP for measurement of LDH activity in extracts.

SOP for measurement of PFK activity in extracts.

SOP for measurement of PGI activity in extracts.

SOP for measurement of PGK activity in extracts.

SOP for measurement of PGM activity in extracts.

SOP for measurement of PGM activity in extracts.

SOP for measurement of PK activity in extracts.

Preparation of cell free extracts of the recombinant E. coli strains expressing the gluconeogenic S. solfataricus enzymes.

Purification of gluconeogenic enzymes from S. solfataricus in recombinant E.coli extracts

SOP for measurement of TPI activity in extracts.

SOP for the isolation of intact Plasmodium falciparum trophozoites from infected red blood cells and the preparation of a cell free extract that can be used for kinetic analyses.

SOP for the determination of external metabolites (Glc, Pyr, Gly, Lac) in intact trophozoite incubations, and for the determination of intracellular metabolite concentrations.

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