Katja Bettenbrock

About Katja Bettenbrock:

I am interested in the coupling of global regulation and metabolism in E. coli. To analyze this I construct and analyze defined mutant strains. These strains are characterized in bioreactor experiments of different types (batch, conti, pulse ...) and measurements on the level of metabolites, mRNA, and protein are applied. For all projects there are cooperation partners that use the data in modeling approaches either from the MPI Magdeburg or from the SUMO consortium.

SEEK ID: https://fairdomhub.org/people/31

Location: Germany

Expertise: Regulatory Networks, Molecular Biology, Genetics, Microbiology

Tools: Mutant and Strain Construction; RNA / DNA Techniques; RT-RealTime-PCR; 2D-Gels; Metabolite Analysis; Bioreactor Experiments; Flow Cyometry; ...

ORCID: Not specified

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Project positions

Other Projects: SUMO (Max Planck Institute for Dynamics of Complex Technical Systems) ; EraCoBiotech 2 nd call proposal preparation (Max Planck Institute for Dynamics of Complex Technical Systems)

Glycon proposal preparation

Programme: Model repository for M4 (Make Me My Model) clients of ISBE

Public web page: Not specified

Organisms: Sulfobacillus thermosulfidooxidans

SUMO

"Systems Understanding of Microbial Oxygen responses" (SUMO) investigates how Escherichia coli senses oxygen, or the associated changes in oxidation/reduction balance, via the Fnr and ArcA proteins, how these systems interact with other regulatory systems, and how the redox response of an E. coli population is generated from the responses of single cells. There are five sub-projects to determine system properties and behaviour and three sub-projects to employ different and complementary modelling
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Programme: SysMO

Public web page: http://www.sysmo.net/index.php?index=55

Organisms: Escherichia coli, Escherichia coli K-12

Max Planck Institute for Dynamics of Complex Technical Systems

Country: Germany

City: Magdeburg

Web page: http://www.mpi-magdeburg.mpg.de

Analysis of population heterogeneity in chemostat cultures with limited oxygen supply

SUMO

Cultures grown under standard SUMO conditions were analyzed with respect to heterogeneity in gene expression. To this end GFP reporter strains were constructed and GFP expression at single cell level was monitored by flow cytometry.

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Studies: No Studies

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Created: 20th May 2014 at 08:22

Analysis of Escherichia coli with linear electron transport chain

SUMO

The electron transport chain of E. coli is branched. Different NAD Dehydrogenases and terminal oxidases are known to be expressed at different oxygen availabilities. By deleting multiple genes mutant strains were constructed that posses a linear electron transport chain. These mutants were investigated in continous bioreactor experiments with limiting glucose and varying oxygen supply.

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Studies: Analysis of Escherichia coli strains with linear respiratory chain

Assays: Determination of by-product formation and glucose uptake of mutants with..., Deternination of ArcA phosphroylation level in mutants with linear ETC a..., Gene expression analysis of mutants with linear electron transport chain...

Created: 14th May 2014 at 09:53

Analysis of Escherichia coli strains with linear respiratory chain

SUMO

A set of isogenic mutant strains was constructed which lack NADH Dehydrogenase I as well as two terminal oxidases, resulting in strains with linear respiratory chain. The different strains hence differ in the terminal oxidase and express either cytochrome bo, cytochrome bdI or cytochrome bdII.
The different strains were cultivated in glucose-limited chemostats with defined low levels of oxygen supply. Biomass and by-product formation, gene expression and the phosphorylation state of the important
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Person responsible: Katja Bettenbrock

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Investigation: Analysis of Escherichia coli with linear electr...

Created: 15th May 2014 at 11:49

Determination of by-product formation and glucose uptake of mutants with linear respiratory chain at different aerobiosis levels

SUMO

Mutants with a linear respiratory chain consisting of NADH Dehydrogenase II and one of the terminal oxidases cytochrom bo, cytochrome bd I or cytochrome bd II were growth in chemostats with defined oxygen supply. The amounts of biomass formed and of acetate and formate produced were determined.

Katja Bettenbrock

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