Katja Bettenbrock
I am interested in the coupling of global regulation and metabolism in E. coli. To analyze this I construct and analyze defined mutant strains. These strains are characterized in bioreactor experiments of different types (batch, conti, pulse ...) and measurements on the level of metabolites, mRNA, and protein are applied. For all projects there are cooperation partners that use the data in modeling approaches either from the MPI Magdeburg or from the SUMO consortium.
SEEK ID: https://fairdomhub.org/people/31
Location:
Germany
Expertise: Regulatory Networks, Molecular Biology, Genetics, Microbiology
ORCID: Not specified
Project roles
Asset housekeeper
Katja's tagsRelated items
Glycon proposal preparation
Programme: Model repository for M4 (Make Me My Model) clients of ISBE
Public web page: Not specified
"Systems Understanding of Microbial Oxygen responses" (SUMO) investigates how Escherichia coli senses oxygen, or the associated changes in oxidation/reduction balance, via the Fnr and ArcA proteins, how these systems interact with other regulatory systems, and how the redox response of an E. coli population is generated from the responses of single cells. There are five sub-projects to determine system properties and behaviour and three sub-projects to employ different and complementary modelling
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Programme: SysMO
Public web page: http://www.sysmo.net/index.php?index=55
Cultures grown under standard SUMO conditions were analyzed with respect to heterogeneity in gene expression. To this end GFP reporter strains were constructed and GFP expression at single cell level was monitored by flow cytometry.
Snapshots: No snapshots
The electron transport chain of E. coli is branched. Different NAD Dehydrogenases and terminal oxidases are known to be expressed at different oxygen availabilities. By deleting multiple genes mutant strains were constructed that posses a linear electron transport chain. These mutants were investigated in continous bioreactor experiments with limiting glucose and varying oxygen supply.
Snapshots: No snapshots
A set of isogenic mutant strains was constructed which lack NADH Dehydrogenase I as well as two terminal oxidases, resulting in strains with linear respiratory chain. The different strains hence differ in the terminal oxidase and express either cytochrome bo, cytochrome bdI or cytochrome bdII.
The different strains were cultivated in glucose-limited chemostats with defined low levels of oxygen supply. Biomass and by-product formation, gene expression and the phosphorylation state of the important
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Person responsible: Katja Bettenbrock
Snapshots: No snapshots
Mutants with a linear respiratory chain consisting of NADH Dehydrogenase II and one of the terminal oxidases cytochrom bo, cytochrome bd I or cytochrome bd II were growth in chemostats with defined oxygen supply. The amounts of biomass formed and of acetate and formate produced were determined.
Contributor: Katja Bettenbrock
Assay type: Experimental Assay Type
Technology type: Chemostat Measurement
Snapshots: No snapshots
Mutant strains with linear electron transport chain were grown in chemostat cultures at different defined aerobiosis levels. Expression of selected genes was determined by Real Time RT-PCR
Contributor: Katja Bettenbrock
Assay type: Experimental Assay Type
Technology type: qRT-PCR
Snapshots: No snapshots
Mutants with linear respiratory chains were grown under SUMO chemostat conditions at different defined aerobiosis levels. The ArcA phoshorylation state as determined.
Contributor: Katja Bettenbrock
Assay type: Experimental Assay Type
Technology type: Gel Electrophoresis
Snapshots: No snapshots
Contributor: Katja Bettenbrock
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Escherichia coli k-12
Genotypes: del ptsG;del manXYZ
Phenotypes: wild-type
Comment: Not specified
Contributor: Katja Bettenbrock
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Escherichia coli k-12
Genotypes: del ptsG;del malEFG
Phenotypes: wild-type
Comment: Not specified
Contributor: Katja Bettenbrock
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Escherichia coli k-12
Genotypes: del ptsG;del mglBAC;del galP
Phenotypes: wild-type
Comment: Not specified
Contributor: Katja Bettenbrock
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Escherichia coli k-12
Genotypes: del ptsG;del mglBAC
Phenotypes: wild-type
Comment: Not specified
Contributor: Katja Bettenbrock
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Escherichia coli k-12
Genotypes: del ptsG
Phenotypes: slow growth with glucose
Comment: Not specified
Contributor: Katja Bettenbrock
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Escherichia coli k-12
Genotypes: del manXYZ
Phenotypes: wild-type
Comment: Not specified
Contributor: Katja Bettenbrock
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Escherichia coli k-12
Genotypes: del malEFG
Phenotypes: wild-type
Comment: Not specified
Contributor: Katja Bettenbrock
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Escherichia coli k-12
Genotypes: del mglBAC;del galP
Phenotypes: wild-type
Comment: Not specified
Contributor: Katja Bettenbrock
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Escherichia coli k-12
Genotypes: del galP
Phenotypes: wild-type
Comment: Not specified
Contributor: Katja Bettenbrock
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Escherichia coli k-12
Genotypes: del mglBAC
Phenotypes: wild-type
Comment: Not specified
This data file shows results from the different chemostat experiments.Gene expression rates are presented.
Creators: Katja Bettenbrock, Sonja Steinsiek
Contributor: Katja Bettenbrock
Downloadby-product formation rates and glucose uptake rates of mutants with linear electron transport chain at different aerobiosis levels
Creator: Katja Bettenbrock
Contributor: Katja Bettenbrock
The ArcA phosphorylation state was determined for mutants with linear respiratory chain at defined aerobiosis levels.
Creator: Katja Bettenbrock
Contributor: Katja Bettenbrock
Gene expression levels in mutants with linear ETC were determined by RealTime RT-PCR
Creator: Katja Bettenbrock
Contributor: Katja Bettenbrock
Abstract (Expand)
Authors: None
Date Published: 27th Mar 2014
Journal: Front Microbiol
Abstract (Expand)
Authors: None
Date Published: 30th Sep 2014
Journal: PLoS One
Abstract (Expand)
Authors: Katja Bettenbrock, Hao Bai, Michael Ederer, Jeff Green, Klaas Hellingwerf, Michael Holcombe, Matthew Rolfe, Guido Sanguinetti, Oliver Sawodny, Poonam Sharma, Sonja Steinsiek, Robert Poole, Kunz S.
Date Published: 7th May 2014
Journal: Adv Microb Physiol
Abstract (Expand)
Author: Sharma P.,Stagge S.,Bekker M.,Bettenbrock K.,Hellingwerf K. J.
Date Published: 7th Oct 2013
Journal: PLoS One
Abstract (Expand)
Authors: None
Date Published: 27th Jan 2014
Journal: PLoS One
Abstract (Expand)
Authors: None
Date Published: 8th Oct 2012
Journal: Journal of Bacteriology
Abstract:
Authors: Sonja Steinsiek, Stefan Stagge, Katja Bettenbrock, Frixel S.
Date Published: 1st Jun 2011
Journal: Journal of Biotechnology
