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Aerobic and anaerobic characterization of MG1655 and mutant strains with regard to growth, by-product formation and cAMP concentrations.

SUMO

Glucose transporter mutants were analyzed under aerobic and aerobic conditions in batch cultures with glucose as substrate. Acetate formation rates and glucose consumption rates were measured, as well as extracellular cAMP concentrations.

Submitter: Sonja Steinsiek

Assay type: Extracellular Metabolite Concentration

Technology type: Technology Type

Investigation: Analysis of the glucose transport in Escherichi...

Study: Characterization of mutant strains with defects...

Organisms: Escherichia coli : MG1655 (del LAM;del rph-1 / pyrimidine starvation ) (batch)

SOPs: Measurement of extracellular metabolites and 1 hidden item

Data files: Growth, by-product formation and glucose consum..., Growth, yield and cAMP excretion under anaerobi...

Snapshots: No snapshots

Created: 17th Jan 2013 at 12:18, Last updated: 8th Nov 2017 at 14:21

Analysis of by-product formation rates in MG1655

SUMO

This document describes by-product formation rates measured in MG1655 at steady-state conditions in Infors-Multifors-Bioreactors.

Submitter: Sonja Steinsiek

Assay type: Metabolomics

Technology type: Technology Type

Investigation: Steady state studies for different oxygen avail...

Study: Determination of the impact of specific enzyme ...

Organisms: Escherichia coli (chemostat)

SOPs: DCW measurement, Evans Medium, Measurement of extracellular metabolites, SUMO chemostat conditions

Data files: 3 hidden items

Snapshots: No snapshots

Created: 21st Oct 2009 at 12:05, Last updated: 8th Nov 2017 at 14:21

Analysis of gene expression rates at different aerobiosis levels via RT-PCR

SUMO

This assay describes how to analyze gene expression rates via RT-PCR.

Submitter: Sonja Steinsiek

Assay type: Gene Expression Profiling

Technology type: qRT-PCR

Investigation: Steady state studies for different oxygen avail...

Study: Determination of the impact of specific enzyme ...

Organisms: Escherichia coli (chemostat)

SOPs: Evans Medium, SUMO chemostat conditions, SUMO mRNA isolation Epicentre

Data files: Gene expression rates at different aerobiosis l... and 3 hidden items

Snapshots: No snapshots

Created: 21st Oct 2009 at 11:50, Last updated: 8th Nov 2017 at 14:21

ArcA phosphorylation at different aerobiosis levels (steady states)

SUMO

ArcA phosphorylation in chemostat cultures grown at different aerobiosis levels was quantitated by Phos-tag SDS-PAGE gel analysis and subsequent immunodetection of ArcA.

Submitter: Matthew Rolfe

Assay type: Experimental Assay Type

Technology type: SDS-PAGE

Investigation: Steady state studies for different oxygen avail...

Study: The Escherichia coli steady state response to o...

Organisms: Escherichia coli : MG1655 (del LAM;del rph-1 / pyrimidine starvation ) (chemostat)

SOPs: Determination of protein phosphorylation levels..., Evans Medium, SUMO chemostat conditions

Data files: No Data files

Snapshots: No snapshots

Created: 30th Jan 2013 at 15:22, Last updated: 8th Nov 2017 at 14:21

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