The principles of Stealthy Engineering (Adamczyk et al.: Biotechnology Journal 2012; 7(7):877-83) are illustrated in this model by emulating a cross engineering intervention between L. lactis and S. cerevisiae.
The case study consists of replacing the native glucose uptake system of L. lactis with that native to the yeast S. cerevisiae. A modified version of Hoefnagel et al.’s model of L. lacrtis’ central metabolism was used as starting point. The total functional replacement of the PTS with the yeast glucose transport plus hexokinase was achieved by modifying the relevant parameters according to the Stealthy Engineering approach discussed in Adamczyc et al. 2012.
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Created: 9th Jan 2013 at 15:20
Last updated: 12th May 2016 at 16:25
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