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All spots and identified proteins of Clostridium acetobutylicum growing at steady-state pH 5.7 (acidogenesis) and pH 4.5 (solventogenesis), respectively, using 2D PAGE and Maldi-TOF analysis. The focus were cytosolic proteins with an isoelectric point bewteen 4 and 7 as well as a molecular weight of 180-10 kDa.
Proteomics data comparing LXX cultures cultivated on mineral and in continuous culture:
conitnuous culture samples:
This template is a JERM compliant spreadsheet for use with gel electrophoresis data. It is in the style of the JERM MASTER template with worksheets for metadata, organism_sample, instrument and data. To conform to MIAPE-GE all worksheets are mandatory.
A detailed description of MIAPE-GE is available from http://www.psidev.info/miape/MIAPE_GE_1_4.pdf
An example of a blank data sheet for Mass spectrometry data. This is not SysMO specific, but it is an example of MIAPE compliant data.
The shreadsheet contains macros to help you annotate your data with terms from controlled vocabularies and ontologies