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No description specified

Creator: Tomas Fiedler

Contributor: JERM

Metabolic changes of 26 intracellular metabolites extracted from T. b. brucei 427 wild type and arginine kinase knockout cells exposed to methylene blue

Metabolic changes of 26 intracellular metabolites extracted from T. b. brucei 427 wild type and arginine kinase knockout cells under high pH stress (pH8.7)

Intracellular concentrations of 50 metabolites measured by LC-MS using isotope ratio based mass spectrometry technique

The Table represents the simulation results of how the presence of phosphate ions (Pi) in the solution might affect the activity of four LDH enzymes. This includes the algorithmic analysis of the binding energies values computed by the GRID program (see Part 4, model) for each enzyme in presence and absence of FBP molecule at pH 6 and 7. The analysis was performed by using the algorithm proposed in Part 5, model.

Activity assays in cell free extracts of S. solfataricus grown on either L-fucose or D-glucose and activity assays with recombinant proteins

TbTryS activity was measured at 37°C in the in vivo-like buffer. All substrate stock solutions were prepared in the in vivo-like buffer and the pH was adjusted to 7.0. The assays were performed in a final volume of 2 ml and contained 0.2 mM NADH, 1 mM phosphoenolpyruvate, 4 units pyruvate kinase, 4 units L-lactate dehydrogenase, 0.17 µM TbTryS, 2.1 mM ATP and varying amounts of GSH, and Spd.

The file contains the initial rate measurements of TbTryS obtained under different substrate and product initial concentrations.

Agenda for the satellite data management tutorial of Synthetic Biology 2017.

Creator: Natalie Stanford

Contributor: Natalie Stanford

3rd ERASysAPP – EXCHANGE Day: Networking and Info Day for research projects of the first and second ERASysAPP calls

Creators: Olga Krebs, Katalin Zsuzsanna Nagy, Heide Marie Hess

Contributor: Olga Krebs

Final version of the Agenda: All Hands PALs meeting on 21-22 of May 2012 in Warnemünde/Rostock.

Creators: None

Contributor: Olga Krebs

Experimental data set for the kinetic characterisation of ALD

raw data OD600

Creator: Koen Van Grinsven

Contributor: Koen Van Grinsven

amino acid auxotrophies as determined for S. pyogenes, E. faecalis, L. lactis and L. plantarum (ATCC, NC8, JDM1 and WCFS1)
subsequent inoculation (3x) in CDM

Creator: Koen Van Grinsven

Contributor: Koen Van Grinsven

raw data OD600 of amino acid auxotrophy exps S. pyogenes

Creator: Koen Van Grinsven

Contributor: Koen Van Grinsven

amino acid auxotrophy for streptococcus pyogenes in CDM-IMIKRO (not CDM-LAB) as function of final growth yield (OD600 at stationary phase) in 96well plates (200 ul culture)

Sulfolobus solfataricus P2 was cultivated on 1 % Caseinhydrolysate. Samples of the culture supernatants were taken at regular intervalls and analyzed by GC-MS. To evaluate the stability of amino acids under cultivation conditions an additional non-inoculated control culture was also analyzed.

Creators: Jacqueline Wolf, Dietmar Schomburg, Meina Neumann-Schaal, Julia Hofmann

Contributor: Jacqueline Wolf

No description specified

Creator: Margrete Solheim

Contributor: Margrete Solheim

No description specified

Creators: John Raedts, Wouter Kuit

Contributor: John Raedts

S. pneumoniae Control1 DNA-Seq PE1

Creator: Jan-Willem Veening

Contributor: Jan-Willem Veening

S. pneumoniae Control 1 DNA PE2

Creator: Jan-Willem Veening

Contributor: Jan-Willem Veening

S. pneumoniae Control 2 DNA PE1

Creator: Jan-Willem Veening

Contributor: Jan-Willem Veening

S. pneumoniae Control 2 DNA PE2

Creator: Jan-Willem Veening

Contributor: Jan-Willem Veening

S. pneumoniae HPUra 1 DNA PE1

Creator: Jan-Willem Veening

Contributor: Jan-Willem Veening

S. pneumoniae HPUra 1 DNA PE2

Creator: Jan-Willem Veening

Contributor: Jan-Willem Veening

S. pneumoniae HPUra 2 DNA PE1

Creator: Jan-Willem Veening

Contributor: Jan-Willem Veening

S. pneumoniae HPUra 2 DNA PE2

Creator: Jan-Willem Veening

Contributor: Jan-Willem Veening

S. pneumoniae kanamycin DNA PE1

Creator: Jan-Willem Veening

Contributor: Jan-Willem Veening

S. pneumoniae kanamycin DNA PE2

Creator: Jan-Willem Veening

Contributor: Jan-Willem Veening

E. coli DNA-Seq
Barcodes:
control 1: GTGGCC
control 2: GTTTCG
Trimethoprim 1: CACTCA
Trimethoprim 2: CAGGCG

Creator: Jan-Willem Veening

Contributor: Jan-Willem Veening

E. coli RNA-Seq
Barcodes:
Control 1: AGTTCC
Control 2: ATGTCA

Creator: Jan-Willem Veening

Contributor: Jan-Willem Veening

E. coli RNA-Seq
Barcodes:
Trimethoprim 1: CCGTCC
Trimethoprim 2: GTAGAG

Creator: Jan-Willem Veening

Contributor: Jan-Willem Veening

S. pneumoniae RNA-Seq
Barcodes:
Control 1: CAGATC
Control 2: ACTTGA

Creator: Jan-Willem Veening

Contributor: Jan-Willem Veening

S. pneumoniae RNA-Seq
Barcodes:
HPUra 1: CAGATC
HPUra 2: ACTTGA
Kanamycin: AGTCAA

Creator: Jan-Willem Veening

Contributor: Jan-Willem Veening

This document shows the ArcA phosphorylation levels at different aerobiosis units, measured using Phos-tag gels, western immunoblotting. Exposed films were quantitated using ImageJ and the results shown here.

Creator: Matthew Rolfe

Contributor: Matthew Rolfe

No description specified

Creator: Maike Bartholomae

Contributor: Maike Bartholomae

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