The file contains the normalized relative read counts (RPM) of 2 mRNA decay experiments. Columns in blue correspond to experiment 1, columns in violet correspond to experiment 2. The time points are in column headers. The last 3 columns contain parameters and half lives calculated from an exponantial fit of all data points. Normalization was done in 2 steps :first by calculating RPM i.e. reads per million of aligned reads to unique ORFs, second by normalizing this to the total amount of mRNA detected according to northern blot hibridization signal to the splice leader sequence. After that, all time point data were calculated in reference to time point 0.
Views: 2642 Downloads: 96
Created: 10th May 2012 at 15:28
Last updated: 20th Nov 2014 at 09:30
Last used: 26th Jun 2019 at 00:16