Whole genome marker frequency analysis on strain MK422

BuildDownload ZoomView content

Strain MK422 was grown under the same conditions as used when growing cells for microscopy analysis; cells of OD600 = 0.4 were diluted 100-fold in C+Y medium with 0.1 mM ZnCl2 and incubated for 2.5 hours until OD600 = 0.15. Cells were then harvested by centrifugation for 5 min at 6500 x g at 4°C. Genomic DNA was isolated using the Wizard® Genomic DNA Purification Kit (Promega) as described previously (Slager et al. 2014 Cell). Fragmentation was performed using Covaris instrument, and libraries were prepared using NEBNext Ultra DNA Library Kit for Illumina prior to sequencing on an Illuminia HiSeq 2000 Platform (all performed at the Genomics Core Facility, EMBL, Heidelberg, Germany).

FAIRDOMHub ID: https://seek.sysmo-db.org/data_files/1430?version=1

Filename: C74M6ACXX_MK422_15s010990-1-1_Aprianto_lane215s010990_sequence.txt

Format: Plain text document

Size: 1.66 GB

help Contributor and Creators
Activity

Views: 630   Downloads: 24

Created: 19th Jul 2016 at 10:22

Last used: 22nd Sep 2017 at 18:35

help Attributions

None

Related items

Powered by
Seek new full
(v.1.4.1)
Copyright © 2008 - 2017 The University of Manchester and HITS gGmbH