Genes are transcribed in polysictronic messages (pre-mRNA) that are destined for either maturation into mRNAs, or degradation. Since transcription regulation is non-existent with few exceptions, the rate of pre-mRNA processing, together with mRNA decay and translation rates, are believed to control gene expression. In this assay, 2T1 blood form trypanosomes are subject to treatment by ActinomycinD for 5 minutes, inhibiting transcription. The cells are harvested, depleted for ribosomal RNA, and sent for deep sequencing. 2 biological replicates have been processed. Reads that align to 20 bp just upstream of 5' splice sites, are believed to correspond to pre-mRNA. The number of reads is compared to a control sample, and the rate of processing is calculated assuming an exponential profile.
The results for blood form Trypanosomes will be compared to the results from 2 replicates for the procyclic form. Differences will be linked to differences in mature mRNA abundances between the two forms.
SEEK ID: https://fairdomhub.org/assays/235
Investigation: Gene expression in Trypanosoma brucei
Assay type: Transcriptomics
Technology type: Rna-seq
Created: 17th Sep 2013 at 12:07
Last updated: 8th Nov 2017 at 15:21