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Measurements of acetone, butanol, acetate, butyrate and ethanol taken during dynamic shift (pH 5.8, 5.5, 5.3, 5.1, 4.9, 4.7, 4.5) and at steady state (pH 5.7, 5.5, 5.3, 5.1, 4.9, 4.7, 4.5).
SEEK ID: https://fairdomhub.org/assays/121
Experimental Assay
Projects: COSMIC
Investigation: The effect of pH upon the metabolic shift in Clostridium acetobutylicum in continuous culture.
Study: Effect of pH on the metabolome
Assay type: Metabolomics
Technology type: Technology Type
Organisms: Clostridium acetobutylicum : ATCC824 (wild-type / wild-type) (chemostat)
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Created: 8th Nov 2010 at 16:41
Last updated: 8th Nov 2017 at 15:21
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Projects: COSMIC
Institutions: University of Nottingham
I am a Birmingham and MRC Fellow in mathematical biology. Specialising in the modelling of gene regulation networks using both numerical and analytical approaches, my work spans a range of biological applications, from drug development to bioenergy to understanding bacterial behaviour. My MRC fellowship gave me the opportunity to gain experimental training in order to generate the complementary data required to adopt a truly interdisciplinary approach to mathematical modelling in biology.
I worked
...
Systems Biology of Clostridium acetobutylicum - a possible answer to dwindling crude oil reserves
Programme: SysMO
Public web page: http://www.sysmo.net/index.php?index=54
Organisms: Clostridium acetobutylicum
Snapshots: No snapshots
Studies: Effect of pH on the metabolome, Effect of pH on the proteome, Effect of pH upon the transcriptome, Identification of clusters of co-regulated and anti-regulated genes, Modelling the effect of pH on the metabolic shift
Assays: Comparison of the proteome between pH 5.7 (acidogenesis) and pH 4.5 (sol..., Comparison of the transcriptome between pH 5.7 (acidogenesis) and pH 4.5..., Comparison of the transcriptome between pH 5.8 (acidogenesis) and pH 4.5..., Identification of dynamically similar transcript profiles, Steady state study of the effect of gene regulation on yields of end-pro..., Study of the end products of the acidogenesis and solventogenesis pathways, Time-dependent simulations
Contributor: Sebastian Curth
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Clostridium acetobutylicum
Genotypes: wild-type
Phenotypes: wild-type
Comment: Not specified
Monitoring of end products ethanol, acetone, butanol, acetate and butyrate during master fermentation of Clostridium acetobutylicum at the steady state pH values 5.5, 5.3, 5.1, 4.9 and 4.7.
Investigations: The effect of pH upon the metabolic shift in Cl...
Studies: Effect of pH on the metabolome
Monitoring of end products in mM of ethanol, acetone, butanol, acetate and butyrate during master fermentation of Clostridium acetobutylicum at the steady state pH values 5.7 (acidogenesis) and 4.5 (solventogenesis).
Investigations: The effect of pH upon the metabolic shift in Cl...
Studies: Effect of pH on the metabolome
Abstract (Expand)
Authors: Holger Janssen, Christina Döring, Armin Ehrenreich, Hubert Bahl, Ralf-Jörg Fischer, Voigt Birgit,Hecker Michael
Date Published: 1st Aug 2010
Journal: Appl Microbiol Biotechnol
DOI: 10.1007/s00253-010-2741-x
Citation: