SEEK ID: https://fairdomhub.org/assays/103
Investigation: Effect of Benzoic Acid on ATP Levels
Assay type: Metabolomics
Technology type: Imaging
Created: 8th Jun 2010 at 06:07
Last updated: 8th Nov 2017 at 15:21
MOSES (Micro Organism Systems biology: Energy and Saccharomyces cerevisiae) develops a new Systems Biology approach, which is called 'domino systems biology'. It uses this to unravel the role of cellular free energy ('ATP') in the control and regulation of cell function. MOSES operates though continuous iterations between partner groups through a new systems-biology driven data-management workflow. MOSES also tries to serve as a substrate for three or more other SYSMO programs.
This investigation examines the effect of benzoic acid, a food preservative, on the ATP levels of yeast cells.
Snapshots: No snapshots
Studies: ATP levels after benzoic acid pretreatments, ATP levels under 10 mM benzoic acid stress, ATP levels under 30 mM and 50 mM benzoic acid stress, Cell survival under different benzoic acid concentrations, Test different ATP extraction protocols and conditions
The kit is used for the quantitative detection of ATP in yeast cells by luciferase driven
bioluminescence. ATP extraction is done by boiling cells in TE buffer.
Investigations: Effect of Benzoic Acid on ATP Levels