Assay type 'Discontinuous enzymatic'
No child terms
TbTryS activity was measured at 37°C in the in vivo-like buffer. All substrate stock solutions were prepared in the in vivo-like buffer and the pH was adjusted to 7.0. The assays were performed in a final volume of 2 ml and contained 0.2 mM NADH, 1 mM phosphoenolpyruvate, 4 units pyruvate kinase, 4 units L-lactate dehydrogenase, 0.17 µM TbTryS, 2.1 mM ATP and varying amounts of glutathione, and spermidine.
Contributor: Alejandro Leroux
Assay type: Discontinuous enzymatic
Technology type: Hplc
Investigation: Kinetic understanding of the T. brucei trypanot...